Korean J Hematol 1994; 29(2):
Published online June 30, 1994
© The Korean Society of Hematology
수혈후 동종면역의 방지책으로서 혈액에 대한 자외선 조사
서철원, 김대원, 지현숙, 성경제, 김미정, 염나미, 조영주, 김상희
울산의대 서울중앙병원 내과학교실,
울산의대 서울중앙병원 임상병리과학교실,
울산의대 서울중앙병원 피부과학교실,
아산생명과학연구소 면역학실,
아산생명과학연구소 세포생물학실
Ultraviolet Irradiation to Blood As a Strategy to Prevent Post-Transfusion Alloimmunization
Background: Platelet(PLT) transfusion is used for prophylaxis and treatment of hemorrhage in patients with thrombocytopenia. Repeated PLT transfusion is known to
cause refractoriness to further PLT transfusion. Refractoriness is almost due to alloimmunization induced by leukocytes contaminated in PLT concentrates.
Therapeutic measure to overcome refractoriness has been developed, but no one is practical and preventive measure is also limited. New preventive measure such as inactivating leukocytes functionally has been searched. Among them ultraviolet(UV) irradiation of leukocyte is expected to be cost-effective.
Methods: UV-B was irradiated to whole blood at 0.005-2.0J/㎠. PLT aggregation test was performed with ADP and collagen before and after UV-B exposure. Loss of immunologic stimulatory function after UV-B irradiation was tested by mixed Iymphocyte culture.
Result : UV-B irradiation to whole blood was feasible. PLT aggregation tests showed no impairment of platelet function after any dose of UV-B in the range of 0.005-2.0J/㎠. Stimulatory index and relative response was somewhat decreased after UV-B irradiation but remained over 40% of the baseline value.
Conclusion: UV-B irradiation to blood is feasible and might be one of the most cost-effective method to prevent alloimmunization after blood transfusion.
Keywords Alloimmunization; Blood Transfusion; Ultraviolet B irradiation;
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Korean J Hematol 1994; 29(2): 207-214
Published online June 30, 1994
Copyright © The Korean Society of Hematology.
수혈후 동종면역의 방지책으로서 혈액에 대한 자외선 조사
서철원, 김대원, 지현숙, 성경제, 김미정, 염나미, 조영주, 김상희
울산의대 서울중앙병원 내과학교실,
울산의대 서울중앙병원 임상병리과학교실,
울산의대 서울중앙병원 피부과학교실,
아산생명과학연구소 면역학실,
아산생명과학연구소 세포생물학실
Ultraviolet Irradiation to Blood As a Strategy to Prevent Post-Transfusion Alloimmunization
Cheolwon Suh, Dae Won Kim, Hyun Sook Chi, Kyung Jeh Sung, Mi Jung Kim, Na Mi Youm, Young Joo Cho, Sang Hee Kim
Department of Medicine, Clinical Pathology, Dermatology, Asan Medical Center, College of Medicine, University of Ulsan
Department of Immunology, Cell Biology, Asan Institute fo Life Sciences
Abstract
Background: Platelet(PLT) transfusion is used for prophylaxis and treatment of hemorrhage in patients with thrombocytopenia. Repeated PLT transfusion is known to
cause refractoriness to further PLT transfusion. Refractoriness is almost due to alloimmunization induced by leukocytes contaminated in PLT concentrates.
Therapeutic measure to overcome refractoriness has been developed, but no one is practical and preventive measure is also limited. New preventive measure such as inactivating leukocytes functionally has been searched. Among them ultraviolet(UV) irradiation of leukocyte is expected to be cost-effective.
Methods: UV-B was irradiated to whole blood at 0.005-2.0J/㎠. PLT aggregation test was performed with ADP and collagen before and after UV-B exposure. Loss of immunologic stimulatory function after UV-B irradiation was tested by mixed Iymphocyte culture.
Result : UV-B irradiation to whole blood was feasible. PLT aggregation tests showed no impairment of platelet function after any dose of UV-B in the range of 0.005-2.0J/㎠. Stimulatory index and relative response was somewhat decreased after UV-B irradiation but remained over 40% of the baseline value.
Conclusion: UV-B irradiation to blood is feasible and might be one of the most cost-effective method to prevent alloimmunization after blood transfusion.
Keywords: Alloimmunization, Blood Transfusion, Ultraviolet B irradiation,
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