Korean J Hematol 2006; 41(4):
Published online December 30, 2006
https://doi.org/10.5045/kjh.2006.41.4.272
© The Korean Society of Hematology
박정일, 김형일, 이현우, 강석윤, 장준호, 박준성, 최진혁, 임호영, 김효철
아주대학교 의과대학 종양혈액내과학교실,
성균관대학교 의과대학 삼성서울병원 혈액종양내과
Background:
Numerous cell surface proteins of leukemia cells such as CD33 and CD52 have been identified as diagnostic and therapeutic targets. Thus the profiling of the cell surface proteome and proteins restricted to specific leukemia(s) can provide a way to identify novel targets for leukemia diagnosis and therapy. However, there is a lack of data pertaining to the comprehensive analysis of surface membrane proteins because there are few effective strategies for profiling surface membrane proteomes.
Methods:We report on the application of quantitative proteomic techniques that incorporate affinity- capture and purification on monomeric avidin columns to identify all biotinylated cell surface proteins from leukemia cell lines.
Results:
An analysis of a subset of biotinylated proteins among the different human leukemia cell lines using matrix-assisted laser desorption ionization and tandem mass spectrometry identified, among others, some widely expressed proteins in leukemia cells, such as CD11a, CD11c, CD18, CD31, CD44, and CD147, as well as a set of proteins identified as chaperone proteins, including HSP90, GRP78, GRP75, HSP70, HSP60 and protein disulfide isomerases. On the basis of their known functional roles, several of these proteins may participate in the progression of leukemogenesis and should be considered as potential markers of leukemia.
Conclusion: Comprehensive profiling of the leukemia cell surface proteome provides an effective approach for the identification of commonly occurring proteins as well as proteins with restricted expression patterns to a specific cell line.
Keywords Proteomic technique, Surface membrane proteome, Biotinylated protein
Korean J Hematol 2006; 41(4): 272-281
Published online December 30, 2006 https://doi.org/10.5045/kjh.2006.41.4.272
Copyright © The Korean Society of Hematology.
박정일, 김형일, 이현우, 강석윤, 장준호, 박준성, 최진혁, 임호영, 김효철
아주대학교 의과대학 종양혈액내과학교실,
성균관대학교 의과대학 삼성서울병원 혈액종양내과
Jung Il Park, Hyoung Il Kim, Hyun Woo Lee, Seok Yun Kang, Jun Ho Jang, Joon Seong Park, Jin Hyuk Choi, Ho Yeong Lim, Hugh Chul Kim
Department of Hematology, Oncology, Ajou University School of Medicine, Suwon,
Division of Hematology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Background:
Numerous cell surface proteins of leukemia cells such as CD33 and CD52 have been identified as diagnostic and therapeutic targets. Thus the profiling of the cell surface proteome and proteins restricted to specific leukemia(s) can provide a way to identify novel targets for leukemia diagnosis and therapy. However, there is a lack of data pertaining to the comprehensive analysis of surface membrane proteins because there are few effective strategies for profiling surface membrane proteomes.
Methods:We report on the application of quantitative proteomic techniques that incorporate affinity- capture and purification on monomeric avidin columns to identify all biotinylated cell surface proteins from leukemia cell lines.
Results:
An analysis of a subset of biotinylated proteins among the different human leukemia cell lines using matrix-assisted laser desorption ionization and tandem mass spectrometry identified, among others, some widely expressed proteins in leukemia cells, such as CD11a, CD11c, CD18, CD31, CD44, and CD147, as well as a set of proteins identified as chaperone proteins, including HSP90, GRP78, GRP75, HSP70, HSP60 and protein disulfide isomerases. On the basis of their known functional roles, several of these proteins may participate in the progression of leukemogenesis and should be considered as potential markers of leukemia.
Conclusion: Comprehensive profiling of the leukemia cell surface proteome provides an effective approach for the identification of commonly occurring proteins as well as proteins with restricted expression patterns to a specific cell line.
Keywords: Proteomic technique, Surface membrane proteome, Biotinylated protein