Korean J Hematol 2006; 41(1):
Published online March 30, 2006
https://doi.org/10.5045/kjh.2006.41.1.28
© The Korean Society of Hematology
고리영, 한진영, 한훈, 김재석, 권혁찬, 김성현, 김효진
동아대학교 의과대학 진단검사의학교실, 내과학교실
Background:
The ability of non-myeloablative allogeneic stem cell transplants to eradicate host neoplastic cells is based on the accumulating evidence of a graft-versus-malignancy (GVM) effect. Stable mixed chimerism (MC) is associated to the lower risk for the development of graft-versus-host diseases (GVHD), but this possibly occurs at the expense of the GVM effect. Therefore, assessment of the chimerism status is critical to allow immune intervention to maintain a state of donor-host tolerance and to prevent loss of the graft.
Methods:
Serial post-transplant peripheral blood samples were collected from 17 patients with various malignant diseases following non-myeloablative allogeneic stem cell transplantation. DNA was amplified from the T-cells, and the polymerase chain reaction (PCR) products were quantified by an automated fluorescent DNA analyzer.
Results:
All 17 patients showed T-cell MC at post-transplant, but this varied in degree and duration, and then 3 patterns emerged. Group 1: 5 patients experienced a short interval of T-cell MC prior to conversion to complete donor chimerism (CC) (median: 25 days). Group 2: 5 patients showed a rapid increase of host cells after a brief MC at a median of 21 days. They never achieved CC, and they relapsed or showed progressive diseases. Group 3: 7 patients showed persistent T-cell MC for 40-50 days, and they subsequently gradually converted to CC after a median of 112 days.
Conclusion:
All the patients achieved T-cell MC in post-transplant, but the CC development differed in frequency and speed. GVHD preceded the onset of T-cell CC in the majority of the patients. Serial engraftment monitoring of the T-cell chimerism status during the first 100 days after non-myeloablative stem cell transplantation is important in aiding the clinical management of such patients.
Keywords Non-myeloablative Allogeneic Stem Cell Transplantation, Chimerism, GVHD, STR Analysis, T-cell
Korean J Hematol 2006; 41(1): 28-35
Published online March 30, 2006 https://doi.org/10.5045/kjh.2006.41.1.28
Copyright © The Korean Society of Hematology.
고리영, 한진영, 한훈, 김재석, 권혁찬, 김성현, 김효진
동아대학교 의과대학 진단검사의학교실, 내과학교실
Ri Yeong Goh, Jin Yeong Han, Hoon Han, Jae Seok Kim, Hyuk Chan Kwon, Sung Hyun Kim, Hyo Jin Kim
Departments of, Laboratory Medicine and, Internal Medicine, Dong, A University College of Medicine, Busan, Korea
Background:
The ability of non-myeloablative allogeneic stem cell transplants to eradicate host neoplastic cells is based on the accumulating evidence of a graft-versus-malignancy (GVM) effect. Stable mixed chimerism (MC) is associated to the lower risk for the development of graft-versus-host diseases (GVHD), but this possibly occurs at the expense of the GVM effect. Therefore, assessment of the chimerism status is critical to allow immune intervention to maintain a state of donor-host tolerance and to prevent loss of the graft.
Methods:
Serial post-transplant peripheral blood samples were collected from 17 patients with various malignant diseases following non-myeloablative allogeneic stem cell transplantation. DNA was amplified from the T-cells, and the polymerase chain reaction (PCR) products were quantified by an automated fluorescent DNA analyzer.
Results:
All 17 patients showed T-cell MC at post-transplant, but this varied in degree and duration, and then 3 patterns emerged. Group 1: 5 patients experienced a short interval of T-cell MC prior to conversion to complete donor chimerism (CC) (median: 25 days). Group 2: 5 patients showed a rapid increase of host cells after a brief MC at a median of 21 days. They never achieved CC, and they relapsed or showed progressive diseases. Group 3: 7 patients showed persistent T-cell MC for 40-50 days, and they subsequently gradually converted to CC after a median of 112 days.
Conclusion:
All the patients achieved T-cell MC in post-transplant, but the CC development differed in frequency and speed. GVHD preceded the onset of T-cell CC in the majority of the patients. Serial engraftment monitoring of the T-cell chimerism status during the first 100 days after non-myeloablative stem cell transplantation is important in aiding the clinical management of such patients.
Keywords: Non-myeloablative Allogeneic Stem Cell Transplantation, Chimerism, GVHD, STR Analysis, T-cell
Seung-Hwan Shin, Ji Yoon Lee, Tae Hyang Lee, So-Hye Park, Seung-Ah Yahng, Jae-Ho Yoon, Sung-Eun Lee, Byung-Sik Cho, Dong-Gun Lee, Yoo-Jin Kim, Seok Lee, Chang-Ki Min, Seok-Goo Cho, Dong-Wook Kim, Jong-Wook Lee, Woo-Sung Min, Chong-Won Park, and Hee-Je Kim
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