Korean J Hematol 2011; 46(1):
Published online March 31, 2011
https://doi.org/10.5045/kjh.2011.46.1.36
© The Korean Society of Hematology
1Department of Laboratory Medicine, CHA Bundang Medical Center, Seongnam, Korea.
2Department of Internal Medicine, CHA Bundang Medical Center, Seongnam, Korea.
3Department of Laboratory Medicine, CHA Kangnam Medical Center, Seoul, Korea.
Correspondence to : Correspondence to Ji Young Huh, M.D., Ph.D. Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Yatap-dong, Bundang-gu, Sungnam 463-712, Korea. Tel: +82-31-780-5451, Fax: +82-31-780-5476, jiyoungh@cha.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Many infections are associated with antiphospholipid antibodies (aPLs). The purpose of this study was to investigate the prevalence, persistence, clinical significance, and characteristics of aPLs in hepatitis B virus (HBV)-infected patients.
This study included 143 patients with HBV infection and 32 healthy individuals as controls. The presence of anticardiolipin antibodies (aCL Ab), anti-β2-glycoprotein I antibodies (β2GPI Ab), and lupus anticoagulant (LA) was assessed.
The total prevalence of aPLs in HBV-infected patients was 12.6% (18 of 143). Of these 18 patients, 15 had low to medium titers of aCL Ab (10 with IgM, 4 with IgG, and 1 with both isotypes). β2GPI Ab and LA were detected in 3 (2.1%) and 2 (1.4%) patients with HBV infection, respectively. In follow-up specimens from 14 patients with elevated levels of aCL Ab or β2GPI Ab, 10 (71.4%) showed the persistent presence of aPLs. No clinical manifestations related to aPLs were identified.
In HBV-infected patients, the most frequently detected antiphospholipid antibodies were IgM aCL Ab, which have a weak association with the clinical manifestations of APS. Unlike the transient presence reported for other infection-associated aPLs, most aPLs were persistently detected over a 12-week period in patients with HBV infection.
Keywords Anticardiolipin antibodies, Anti-β2-glycoprotein I antibodies, Lupus coagulation inhibitor, Hepatitis B virus
Antiphospholipid antibodies (aPLs) are a heterogeneous group of autoantibodies or alloantibodies with an affinity for anionic phospholipids [1]. aPLs occur in patients with antiphospholipid syndrome (APS), systemic lupus erythematosus (SLE), and various other rheumatic diseases. Furthermore, an elevated level of aPLs is recognized as a risk factor for thrombosis [2]. Several studies have suggested that the pathogenic mechanisms of aPLs are related to platelet activation, endothelial cell activation, and activation of the complement cascade [3, 4]; however, their pathogenic functions in patients with autoimmune diseases like SLE and primary APS are not fully understood.
aPLs have also been detected in numerous infectious diseases, including those caused by parvovirus B19, cytomegalovirus, varicella-zoster virus, human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV),
The clinical significance of aPLs associated with various infections is controversial. In many studies, the presence of aPLs associated with infections has been regarded as non-pathogenic [5, 8]; however, in patients with various infections, thrombotic manifestations such as portal vein thrombosis and pulmonary embolism have been reported [10-13].
Recently, several studies showed a higher prevalence of aCL Ab in chronic viral hepatitis patients than in control individuals [14-17]. In the present study, we investigated the prevalence, persistence, clinical significance, and characteristics of aPLs in HBV-infected patients.
The prevalence of aPLs was prospectively determined in HBV-infected patients and healthy controls who visited the Gastroenterology Department of the Bundang CHA hospital between 2008 and 2009. This study included 143 HBV-infected patients, irrespective of their treatment (59 women and 84 men; age range, 16-71 years; mean, 42.7 years), and 32 healthy individuals as controls (13 women and 19 men; age range, 27-65 years; mean, 40 years). All patients with HBV infection tested positive for HBV surface antigen (HBsAg) or HBV DNA and negative for anti-HCV antibody. Informed consent was obtained from all patients. The patients were divided into 2 groups on the basis of the hepatitis B e antigen and antibody (HBeAg, HBeAb) status and serum HBV DNA level: chronic hepatitis B patients (N=97) and patients with inactive HBsAg carrier state (N=46) [18]. Patients with positive HBeAg or high levels of HBV DNA (≥104 copies/mL) were considered chronic hepatitis B patients, and patients with negative HBeAg and low HBV DNA level (<104 copies/mL) were considered inactive HBsAg carriers. All normal controls were negative for HBsAg, anti-HCV antibody, anti-HIV antibody, and antinuclear antibody. Alanine aminotransferase (ALT) levels were normal (5-40 IU/L) in all healthy controls. Thromboembolic complications associated with aPLs were identified through medical record review. The local ethics committee of CHA Bundang Medical Center approved this study.
aCL Ab (IgG and IgM isotype) were measured using a commercial enzyme-linked immunosorbent assay (ELISA) (Zeus Scientific Inc., Raritan, NJ). According to the manufacturer's instructions, the cut-off values for positivity were 20 GPL or MPL (IgG or IgM phospholipid units). Specimens with less than 20 GPL or MPL in the initial ELISA were considered negative. Specimens with greater than 20 GPL or MPL in the initial ELISA were retested in duplicate. The specimens were considered positive, when the value was repeatedly greater than 20 GPL or MPL.
β2GPI Ab (IgG and IgM isotype) were measured using a commercial ELISA (INOVA Diagnostics, Inc., San Diego, CA, USA). According to the manufacturer's instructions, the cut-off values for positivity are 20 SGU or SMU (standard IgG or standard IgM units). Specimens with less than 20 SGU or SMU in the initial ELISA were considered negative. Specimens with greater than 20 SGU or SMU in the initial ELISA result were retested in duplicate. The specimens were considered positive when the value was repeatedly greater than 20 SGU or SMU.
All plasma samples from patients and normal individuals were evaluated for the presence of LA according to the criteria defined by the Subcommittee on LA/antiphospholipid antibody of the International Society of Thrombosis and Haemostasis [19]. LA was measured by performing the dilute Russell's viper venom test using the HemosIL Kit (Instrumentation Laboratory, Milano, Italy).
Statistical analyses were performed using SAS Statistical Analysis Software Version 9.1 (SAS Institute Inc., Cary, NC, USA). A chi-square test was used to determine the difference in the presence of aPLs between the chronic viral hepatitis patient group and the control group.
The total prevalence of aPLs (aCL Ab, β2GPI Ab, or LA) in HBV-infected patients was 12.6% (18 of 143) (Table 1). Among these 18 patients, aPLs were detected in 15 of 97 chronic hepatitis B patients and 3 of 46 inactive HBsAg carrier patients. No clinical manifestations related to aPLs were identified. Among the 143 HBV-infected patients, 15 patients (10.5%) had a low to moderate level of aCL Ab (10 with IgM, 4 with IgG, and 1 with both isotypes) compared to only 1 of the 32 control subjects (3.1%). The difference between the groups was not significant (
The prevalence of β2GPI Ab and the LA activity in the HBV-infected patient group was 2.1% (3 of 143) and 1.4% (2 of 143), respectively. In contrast, none of the healthy controls had elevated levels of β2GPI Ab or LA; however, the difference between the groups was not significant (
Follow-up specimens were obtained from 14 of the 17 patients with elevated levels of aCL Ab or β2GPI Ab (11 with aCL Ab, 2 with anti-β2GPI Ab, and 1 with both aCL Ab and β2GPI Ab). The median follow-up duration was 30 weeks (range, 9-100 weeks). The aPLs persisted in 10 of the 14 patients (71.4%), and β2GPI Ab persisted in all 3 patients with β2GPI Ab (Table 3).
aPLs have been found not only in patients with autoimmune diseases like SLE, but also in patients with various infections [5, 8]. Molecular mimicry is the proposed mechanism for the development of aPLs in infections. Previous reports showed that antigenic determinants shared between the antigens of infectious agents and host tissue might trigger the immune response [20, 21]. However, the mechanisms that lead to the development of aPLs and their possible pathophysiological implications in patients with infections have not been well established.
The reported prevalence of aPLs in infectious patients is variable, in part because of a methodological problem. Currently, the ideal approach for standardized measurement for aCL Ab is under debate [22]. A discrepancy is observed among the different assay kits and methods, particularly in the lower range of antibody levels. Furthermore, one of the critical problems in the standardization process is the absence of a defined cut-off value for positivity. In previous reports, the prevalence of aCL Ab in patients with HBV infection was between 14% and 42% [14, 17, 23], and that of β2GPI Ab was 2% [17] and 7.5% [14]. In the present study, the prevalence of aCL Ab and β2GPI Ab was 10.5% and 2.1%, respectively, which was lower than that in previous reports. The lower prevalence of aCL Ab in the present study may be partly because of a higher cut-off value for positivity than that of previous reports. We used 20 GPL or MPL as the cut-off values, whereas 10 GPL or MPL were used as the cut-off values in the other reports [14, 23]. The prevalence of β2GPI Ab in the present study was similar to those in previous reports.
In most cases, infection-associated aPLs appear temporarily and disappear within 2 or 3 months [24, 25], and except in rare cases, they are unrelated to thrombotic complications. Contrary to previous reports, in the present study, most aCL Ab and β2GPI Ab showed persistence and were present over a 12-week period. We speculated that continuous antigenic stimulation might be associated with persistent aPL production, because in the present study, the prevalence of aCL Ab in chronic hepatitis B patients was higher than that in inactive HBsAg carrier patients (15% vs. 6.5%). Furthermore, it was shown that in a patient with HCV infection and aPLs, elimination of the virus was accompanied by the disappearance of aPLs, and when HCV infection relapsed, aPLs also reappeared [26]. Therefore, aPLs associated with other chronic infections caused by HIV or HCV may also show persistent positivity.
According to a previous report [27], IgM aPLs are associated with the clinical manifestations of APS less often than the IgG isotype is, and the diagnostic criteria for APS include only a medium or high titer of aCL Ab (>40 GPL or MPL) [28]. In the present study, IgM aCL Ab were found more frequently (11 of 15) than the IgG isotype (5 of 15), and the titers of aCL Ab were mostly low. These findings support that infection-associated aPLs rarely manifest with the clinical features of APS. Because the rates of aCL Ab according to isotype were not provided in previous reports of aPLs in patients with HBV infection, comparison with our results was not possible.
In conclusion, the most frequently detected aPLs in HBV-infected patients were IgM aCL Ab, which has a weak association with the clinical manifestations of APS. Unlike the transient presence of other infection-associated aPLs, most aPLs were persistently detected over a 12-week period in patients with HBV infection.
Distribution of anticardiolipin antibody titers in hepatitis B virus (HBV)-infected patients and normal controls, IgM isotype
Table 1 Prevalence of antiphospholipid antibodies (aCL Ab, β2GPI Ab, and lupus anticoagulant) in HBV-infected patients.
Abbreviations: HBV, hepatitis B virus; aCL Ab, anticardiolipin antibodies; β2GPI Ab, anti-β2-glycoprotein I antibodies.
Table 2 Characteristics of HBV-infected patients with antiphospholipid antibodies and the types of antiphospholipid antibodies.
a)Patients were divided into 2 groups on the basis of the hepatitis B e antigen and antibody status and the HBV DNA level: chronic hepatitis B patients and patients with inactive HBsAg carrier state.
Abbreviations: HBV, hepatitis B virus; M, male; F, female; aCL, anticardiolipin antibodies; β2GPI, anti-β2-glycoprotein I antibodies; LA, lupus anticoagulant.
Table 3 Follow-up duration and results of aCL Ab and β2GPI Ab testing in antiphospholipid antibody-positive HBV-infected patients.
a)Follow-up specimens of 3 patients (134, 140, and 153) could not be obtained.
Abbreviations: HBV, hepatitis B virus; aCL Ab, anticardiolipin antibodies; β2GPI Ab, anti-2-glycoprotein I antibodies; MPL, IgM phospholipid unit; GPL, IgG phospholipid unit; SMU, standard IgM unit; SGU, standard IgG unit;-, negative; NT, not tested.
Korean J Hematol 2011; 46(1): 36-40
Published online March 31, 2011 https://doi.org/10.5045/kjh.2011.46.1.36
Copyright © The Korean Society of Hematology.
Ji Young Huh1*, Dae Young Yi1, Seong Gyu Hwang2, Jin Jung Choi2, and Myung Seo Kang3
1Department of Laboratory Medicine, CHA Bundang Medical Center, Seongnam, Korea.
2Department of Internal Medicine, CHA Bundang Medical Center, Seongnam, Korea.
3Department of Laboratory Medicine, CHA Kangnam Medical Center, Seoul, Korea.
Correspondence to: Correspondence to Ji Young Huh, M.D., Ph.D. Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Yatap-dong, Bundang-gu, Sungnam 463-712, Korea. Tel: +82-31-780-5451, Fax: +82-31-780-5476, jiyoungh@cha.ac.kr
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Many infections are associated with antiphospholipid antibodies (aPLs). The purpose of this study was to investigate the prevalence, persistence, clinical significance, and characteristics of aPLs in hepatitis B virus (HBV)-infected patients.
This study included 143 patients with HBV infection and 32 healthy individuals as controls. The presence of anticardiolipin antibodies (aCL Ab), anti-β2-glycoprotein I antibodies (β2GPI Ab), and lupus anticoagulant (LA) was assessed.
The total prevalence of aPLs in HBV-infected patients was 12.6% (18 of 143). Of these 18 patients, 15 had low to medium titers of aCL Ab (10 with IgM, 4 with IgG, and 1 with both isotypes). β2GPI Ab and LA were detected in 3 (2.1%) and 2 (1.4%) patients with HBV infection, respectively. In follow-up specimens from 14 patients with elevated levels of aCL Ab or β2GPI Ab, 10 (71.4%) showed the persistent presence of aPLs. No clinical manifestations related to aPLs were identified.
In HBV-infected patients, the most frequently detected antiphospholipid antibodies were IgM aCL Ab, which have a weak association with the clinical manifestations of APS. Unlike the transient presence reported for other infection-associated aPLs, most aPLs were persistently detected over a 12-week period in patients with HBV infection.
Keywords: Anticardiolipin antibodies, Anti-β2-glycoprotein I antibodies, Lupus coagulation inhibitor, Hepatitis B virus
Antiphospholipid antibodies (aPLs) are a heterogeneous group of autoantibodies or alloantibodies with an affinity for anionic phospholipids [1]. aPLs occur in patients with antiphospholipid syndrome (APS), systemic lupus erythematosus (SLE), and various other rheumatic diseases. Furthermore, an elevated level of aPLs is recognized as a risk factor for thrombosis [2]. Several studies have suggested that the pathogenic mechanisms of aPLs are related to platelet activation, endothelial cell activation, and activation of the complement cascade [3, 4]; however, their pathogenic functions in patients with autoimmune diseases like SLE and primary APS are not fully understood.
aPLs have also been detected in numerous infectious diseases, including those caused by parvovirus B19, cytomegalovirus, varicella-zoster virus, human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV),
The clinical significance of aPLs associated with various infections is controversial. In many studies, the presence of aPLs associated with infections has been regarded as non-pathogenic [5, 8]; however, in patients with various infections, thrombotic manifestations such as portal vein thrombosis and pulmonary embolism have been reported [10-13].
Recently, several studies showed a higher prevalence of aCL Ab in chronic viral hepatitis patients than in control individuals [14-17]. In the present study, we investigated the prevalence, persistence, clinical significance, and characteristics of aPLs in HBV-infected patients.
The prevalence of aPLs was prospectively determined in HBV-infected patients and healthy controls who visited the Gastroenterology Department of the Bundang CHA hospital between 2008 and 2009. This study included 143 HBV-infected patients, irrespective of their treatment (59 women and 84 men; age range, 16-71 years; mean, 42.7 years), and 32 healthy individuals as controls (13 women and 19 men; age range, 27-65 years; mean, 40 years). All patients with HBV infection tested positive for HBV surface antigen (HBsAg) or HBV DNA and negative for anti-HCV antibody. Informed consent was obtained from all patients. The patients were divided into 2 groups on the basis of the hepatitis B e antigen and antibody (HBeAg, HBeAb) status and serum HBV DNA level: chronic hepatitis B patients (N=97) and patients with inactive HBsAg carrier state (N=46) [18]. Patients with positive HBeAg or high levels of HBV DNA (≥104 copies/mL) were considered chronic hepatitis B patients, and patients with negative HBeAg and low HBV DNA level (<104 copies/mL) were considered inactive HBsAg carriers. All normal controls were negative for HBsAg, anti-HCV antibody, anti-HIV antibody, and antinuclear antibody. Alanine aminotransferase (ALT) levels were normal (5-40 IU/L) in all healthy controls. Thromboembolic complications associated with aPLs were identified through medical record review. The local ethics committee of CHA Bundang Medical Center approved this study.
aCL Ab (IgG and IgM isotype) were measured using a commercial enzyme-linked immunosorbent assay (ELISA) (Zeus Scientific Inc., Raritan, NJ). According to the manufacturer's instructions, the cut-off values for positivity were 20 GPL or MPL (IgG or IgM phospholipid units). Specimens with less than 20 GPL or MPL in the initial ELISA were considered negative. Specimens with greater than 20 GPL or MPL in the initial ELISA were retested in duplicate. The specimens were considered positive, when the value was repeatedly greater than 20 GPL or MPL.
β2GPI Ab (IgG and IgM isotype) were measured using a commercial ELISA (INOVA Diagnostics, Inc., San Diego, CA, USA). According to the manufacturer's instructions, the cut-off values for positivity are 20 SGU or SMU (standard IgG or standard IgM units). Specimens with less than 20 SGU or SMU in the initial ELISA were considered negative. Specimens with greater than 20 SGU or SMU in the initial ELISA result were retested in duplicate. The specimens were considered positive when the value was repeatedly greater than 20 SGU or SMU.
All plasma samples from patients and normal individuals were evaluated for the presence of LA according to the criteria defined by the Subcommittee on LA/antiphospholipid antibody of the International Society of Thrombosis and Haemostasis [19]. LA was measured by performing the dilute Russell's viper venom test using the HemosIL Kit (Instrumentation Laboratory, Milano, Italy).
Statistical analyses were performed using SAS Statistical Analysis Software Version 9.1 (SAS Institute Inc., Cary, NC, USA). A chi-square test was used to determine the difference in the presence of aPLs between the chronic viral hepatitis patient group and the control group.
The total prevalence of aPLs (aCL Ab, β2GPI Ab, or LA) in HBV-infected patients was 12.6% (18 of 143) (Table 1). Among these 18 patients, aPLs were detected in 15 of 97 chronic hepatitis B patients and 3 of 46 inactive HBsAg carrier patients. No clinical manifestations related to aPLs were identified. Among the 143 HBV-infected patients, 15 patients (10.5%) had a low to moderate level of aCL Ab (10 with IgM, 4 with IgG, and 1 with both isotypes) compared to only 1 of the 32 control subjects (3.1%). The difference between the groups was not significant (
The prevalence of β2GPI Ab and the LA activity in the HBV-infected patient group was 2.1% (3 of 143) and 1.4% (2 of 143), respectively. In contrast, none of the healthy controls had elevated levels of β2GPI Ab or LA; however, the difference between the groups was not significant (
Follow-up specimens were obtained from 14 of the 17 patients with elevated levels of aCL Ab or β2GPI Ab (11 with aCL Ab, 2 with anti-β2GPI Ab, and 1 with both aCL Ab and β2GPI Ab). The median follow-up duration was 30 weeks (range, 9-100 weeks). The aPLs persisted in 10 of the 14 patients (71.4%), and β2GPI Ab persisted in all 3 patients with β2GPI Ab (Table 3).
aPLs have been found not only in patients with autoimmune diseases like SLE, but also in patients with various infections [5, 8]. Molecular mimicry is the proposed mechanism for the development of aPLs in infections. Previous reports showed that antigenic determinants shared between the antigens of infectious agents and host tissue might trigger the immune response [20, 21]. However, the mechanisms that lead to the development of aPLs and their possible pathophysiological implications in patients with infections have not been well established.
The reported prevalence of aPLs in infectious patients is variable, in part because of a methodological problem. Currently, the ideal approach for standardized measurement for aCL Ab is under debate [22]. A discrepancy is observed among the different assay kits and methods, particularly in the lower range of antibody levels. Furthermore, one of the critical problems in the standardization process is the absence of a defined cut-off value for positivity. In previous reports, the prevalence of aCL Ab in patients with HBV infection was between 14% and 42% [14, 17, 23], and that of β2GPI Ab was 2% [17] and 7.5% [14]. In the present study, the prevalence of aCL Ab and β2GPI Ab was 10.5% and 2.1%, respectively, which was lower than that in previous reports. The lower prevalence of aCL Ab in the present study may be partly because of a higher cut-off value for positivity than that of previous reports. We used 20 GPL or MPL as the cut-off values, whereas 10 GPL or MPL were used as the cut-off values in the other reports [14, 23]. The prevalence of β2GPI Ab in the present study was similar to those in previous reports.
In most cases, infection-associated aPLs appear temporarily and disappear within 2 or 3 months [24, 25], and except in rare cases, they are unrelated to thrombotic complications. Contrary to previous reports, in the present study, most aCL Ab and β2GPI Ab showed persistence and were present over a 12-week period. We speculated that continuous antigenic stimulation might be associated with persistent aPL production, because in the present study, the prevalence of aCL Ab in chronic hepatitis B patients was higher than that in inactive HBsAg carrier patients (15% vs. 6.5%). Furthermore, it was shown that in a patient with HCV infection and aPLs, elimination of the virus was accompanied by the disappearance of aPLs, and when HCV infection relapsed, aPLs also reappeared [26]. Therefore, aPLs associated with other chronic infections caused by HIV or HCV may also show persistent positivity.
According to a previous report [27], IgM aPLs are associated with the clinical manifestations of APS less often than the IgG isotype is, and the diagnostic criteria for APS include only a medium or high titer of aCL Ab (>40 GPL or MPL) [28]. In the present study, IgM aCL Ab were found more frequently (11 of 15) than the IgG isotype (5 of 15), and the titers of aCL Ab were mostly low. These findings support that infection-associated aPLs rarely manifest with the clinical features of APS. Because the rates of aCL Ab according to isotype were not provided in previous reports of aPLs in patients with HBV infection, comparison with our results was not possible.
In conclusion, the most frequently detected aPLs in HBV-infected patients were IgM aCL Ab, which has a weak association with the clinical manifestations of APS. Unlike the transient presence of other infection-associated aPLs, most aPLs were persistently detected over a 12-week period in patients with HBV infection.
Distribution of anticardiolipin antibody titers in hepatitis B virus (HBV)-infected patients and normal controls, IgM isotype
Table 1 . Prevalence of antiphospholipid antibodies (aCL Ab, β2GPI Ab, and lupus anticoagulant) in HBV-infected patients..
Abbreviations: HBV, hepatitis B virus; aCL Ab, anticardiolipin antibodies; β2GPI Ab, anti-β2-glycoprotein I antibodies..
Table 2 . Characteristics of HBV-infected patients with antiphospholipid antibodies and the types of antiphospholipid antibodies..
a)Patients were divided into 2 groups on the basis of the hepatitis B e antigen and antibody status and the HBV DNA level: chronic hepatitis B patients and patients with inactive HBsAg carrier state..
Abbreviations: HBV, hepatitis B virus; M, male; F, female; aCL, anticardiolipin antibodies; β2GPI, anti-β2-glycoprotein I antibodies; LA, lupus anticoagulant..
Table 3 . Follow-up duration and results of aCL Ab and β2GPI Ab testing in antiphospholipid antibody-positive HBV-infected patients..
a)Follow-up specimens of 3 patients (134, 140, and 153) could not be obtained..
Abbreviations: HBV, hepatitis B virus; aCL Ab, anticardiolipin antibodies; β2GPI Ab, anti-2-glycoprotein I antibodies; MPL, IgM phospholipid unit; GPL, IgG phospholipid unit; SMU, standard IgM unit; SGU, standard IgG unit;-, negative; NT, not tested..
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Distribution of anticardiolipin antibody titers in hepatitis B virus (HBV)-infected patients and normal controls, IgM isotype