Korean J Hematol 1999; 34(2):
Published online June 30, 1999
© The Korean Society of Hematology
자동혈구분석기로 측정한 미성숙 망상적혈구 분획의 임상적 의의
박경희, 이유경, 최태윤, 김원배, 이동화
순천향대학교 의과대학 임상병리학교실
Clinical Significance of Immature Reticulocyte Fraction Determined by Automated Blood Cell Analyzer
BACKGROUND:
Compared with traditional manual method, automated counting of reticulocytes offers greater sensitivity, improved accuracy, and less subjectivity. Also, automated blood cell analyzer enabled to measure immature reticulocyte fraction (IRF).
In this study, we investigate the clinical significance of IRF in the evaluation of anemia.
METHODS:
Reticulocyte counts were measured with automated blood cell analyzer, Technicon H*3 (Bayer Diagnostics, USA) and manual method from 100 cases of anemic group and 20 cases of control group. Then, we compared the values of reticulocyte counts
between manual method and H*3. For investigate clinical significance of IRF in the evaluation of anemia, IRF determined by H*3 was compared with the absolute reticulocyte count (ARC) and with the reticulocyte production index (RPI). In addition, we
subclassified anemic group into 5 different subgroups, such as hemolytic anemia or anemia due to hemorrhage, hematologic malignancy, anemia due to chronic renal failure, iron deficiency anemia, and anemia of chronic disorders. The value of ARC and IRF
in each group was analysed.
RESULTS:
1) The mean reticulocyte count by H*3 was higher than that by the manual method and the result of these two methods was correlated well (r=0.98, P<0.01).
2) IRF has a weak but significantly positive correlation (P<0.05) with ARC. IRF has insignificant correlation (P>0.05) with RPI.
3) Both ARC and IRF were increased in patients with hemolytic anemia or anemia due to hemorrhage. High mean value of IRF with reduced ARC were shown in hematologic malignancy. IRF were within normal range with reduced ARC in the group of chronic renal
failure. Both IRF and ARC were within normal range in the group of iron deficiency anemia. In anemic group due to chronic disorder, ARC were within normal range with increased IRF.
CONCLUSION:
reticulocyte counting by the automated blood cell analyzer H*3 allows the improvement of accuracy and sensitivity. Immature reticulocyte fraction can be used for differentiation of various anemic disorders together with absolute reticulocyte count.
Keywords Reticulocyte, Technicon H*3, Immature reticulocyte fraction
PDF
Standard view
Export citation
Share Article
Korean J Hematol 1999; 34(2): 281-287
Published online June 30, 1999
Copyright © The Korean Society of Hematology.
자동혈구분석기로 측정한 미성숙 망상적혈구 분획의 임상적 의의
박경희, 이유경, 최태윤, 김원배, 이동화
순천향대학교 의과대학 임상병리학교실
Clinical Significance of Immature Reticulocyte Fraction Determined by Automated Blood Cell Analyzer
Kyung Hee Park, YouKyung Lee, Tae Youn Choi, WonBae Kim, Dong Wha Lee
Department of Clinical Pathology, College of Medicine, Soonchunhyang University, Seoul, Korea
Abstract
BACKGROUND:
Compared with traditional manual method, automated counting of reticulocytes offers greater sensitivity, improved accuracy, and less subjectivity. Also, automated blood cell analyzer enabled to measure immature reticulocyte fraction (IRF).
In this study, we investigate the clinical significance of IRF in the evaluation of anemia.
METHODS:
Reticulocyte counts were measured with automated blood cell analyzer, Technicon H*3 (Bayer Diagnostics, USA) and manual method from 100 cases of anemic group and 20 cases of control group. Then, we compared the values of reticulocyte counts
between manual method and H*3. For investigate clinical significance of IRF in the evaluation of anemia, IRF determined by H*3 was compared with the absolute reticulocyte count (ARC) and with the reticulocyte production index (RPI). In addition, we
subclassified anemic group into 5 different subgroups, such as hemolytic anemia or anemia due to hemorrhage, hematologic malignancy, anemia due to chronic renal failure, iron deficiency anemia, and anemia of chronic disorders. The value of ARC and IRF
in each group was analysed.
RESULTS:
1) The mean reticulocyte count by H*3 was higher than that by the manual method and the result of these two methods was correlated well (r=0.98, P<0.01).
2) IRF has a weak but significantly positive correlation (P<0.05) with ARC. IRF has insignificant correlation (P>0.05) with RPI.
3) Both ARC and IRF were increased in patients with hemolytic anemia or anemia due to hemorrhage. High mean value of IRF with reduced ARC were shown in hematologic malignancy. IRF were within normal range with reduced ARC in the group of chronic renal
failure. Both IRF and ARC were within normal range in the group of iron deficiency anemia. In anemic group due to chronic disorder, ARC were within normal range with increased IRF.
CONCLUSION:
reticulocyte counting by the automated blood cell analyzer H*3 allows the improvement of accuracy and sensitivity. Immature reticulocyte fraction can be used for differentiation of various anemic disorders together with absolute reticulocyte count.
Keywords: Reticulocyte, Technicon H*3, Immature reticulocyte fraction
Previous Article
Article Tools
Stats or Metrics
Share this article on
Related articles in BR
-
Immature Reticulocyte Fraction after Iron Therapy for Iron Deficiency Anemia
Young Jin Yuh, Sung Rok Kim, Tae Hee Han
Korean J Hematol 2004; 39(2): 103-108
