Korean J Hematol 2007; 42(2):
Published online June 30, 2007
https://doi.org/10.5045/kjh.2007.42.2.106
© The Korean Society of Hematology
조현민, 홍종욱, 김준기, 김정아
가톨릭대학교 의과대학, 내과학교실, 조혈모세포 연구실, 외과학교실
Background:
Angiogenesis is enhanced in the ischemic tissues after the injection of bone marrow cells (BMCs). However the exact mechanisms for this are not yet fully understood.
Methods:
A unilateral ischemic limb was surgically induced in mice and then BMCs were injected into the ischemic area. We measured the capillary/muscle ratio. Fluorescence-labeled BMCs were injected into the ischemic tissues and then the locations of the cells were examined by using a confocal microscope. Recruitment of bone marrow-derived cells into the ischemic tissue was examined in a sex-mismatched bone marrow transplantation (BMT) setting by identifying the Y chromosome with using the FISH technique. The expressions of VEGF, MMP-9, SDF-1 and CXCR-4 were measured by Western blot analysis.
Results:
The capillary/muscle ratio was more increased in the BMC-injected group than in the control group (P<0.05). Florescence-labeled BMCs, which had been directly injected into ischemic tissue, were not detected in the tissue. In the sex-mismatched bone marrow transplantation models, the ischemic tissues of the BMC-injected group recruited a much greater number of Y chromosome-positive bone marrow- derived cells, as compared to the control group. The expressions of VEGF and MMP-9 were increased after injection of BMCs. SDF-1 was expressed on the seventh day in the BMC-injected group and CXCR-4 was highly expressed until 12 weeks in the BMC-injected group.
Conclusion:
We suggest that the injection of BMCs into ischemic tissue recruits CXCR-4-positvie cells from the bone marrow via the up-regulation of VEGF, MMP-9 and SDF-1, and these CXCR-4-positive cells may play a role in neovascularization.
Keywords Ischemia, Angiogenesis, Bone marrow cell, VEGF, MMP-9, SDF-1, CXCR-4
Korean J Hematol 2007; 42(2): 106-113
Published online June 30, 2007 https://doi.org/10.5045/kjh.2007.42.2.106
Copyright © The Korean Society of Hematology.
조현민, 홍종욱, 김준기, 김정아
가톨릭대학교 의과대학, 내과학교실, 조혈모세포 연구실, 외과학교실
Hyeon min Cho, Jong Wook Hong, Jun Gi Kim, Jeong A Kim
Department of Internal Medicine, Hematopoietic Stem Cell Transplantation Labaratory,
Department of General Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea
Background:
Angiogenesis is enhanced in the ischemic tissues after the injection of bone marrow cells (BMCs). However the exact mechanisms for this are not yet fully understood.
Methods:
A unilateral ischemic limb was surgically induced in mice and then BMCs were injected into the ischemic area. We measured the capillary/muscle ratio. Fluorescence-labeled BMCs were injected into the ischemic tissues and then the locations of the cells were examined by using a confocal microscope. Recruitment of bone marrow-derived cells into the ischemic tissue was examined in a sex-mismatched bone marrow transplantation (BMT) setting by identifying the Y chromosome with using the FISH technique. The expressions of VEGF, MMP-9, SDF-1 and CXCR-4 were measured by Western blot analysis.
Results:
The capillary/muscle ratio was more increased in the BMC-injected group than in the control group (P<0.05). Florescence-labeled BMCs, which had been directly injected into ischemic tissue, were not detected in the tissue. In the sex-mismatched bone marrow transplantation models, the ischemic tissues of the BMC-injected group recruited a much greater number of Y chromosome-positive bone marrow- derived cells, as compared to the control group. The expressions of VEGF and MMP-9 were increased after injection of BMCs. SDF-1 was expressed on the seventh day in the BMC-injected group and CXCR-4 was highly expressed until 12 weeks in the BMC-injected group.
Conclusion:
We suggest that the injection of BMCs into ischemic tissue recruits CXCR-4-positvie cells from the bone marrow via the up-regulation of VEGF, MMP-9 and SDF-1, and these CXCR-4-positive cells may play a role in neovascularization.
Keywords: Ischemia, Angiogenesis, Bone marrow cell, VEGF, MMP-9, SDF-1, CXCR-4
Ha-Yon Kim, Ji-Young Hwang, Yoon-Suk Oh, Seong-Woo Kim, Hyo-Jin Lee, Hwan-Jung Yun, Samyong Kim, Young-Jun Yang, and Deog-Yeon Jo
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