Diamond Blackfan anemia (DBA; OMIM 205900), characterized by impaired red cell production, is a rare condition that is usually symptomatic in early infancy. DBA was first recognized as a distinct clinical entity in 1938 [1]. The diagnostic criteria for DBA that were developed in 1976 have remained the accepted standard [2] and include anemia, usually presenting prior to the first birthday, with near normal neutrophil and/or platelet counts, reticulocytopenia, macrocytosis, and normal marrow cellularity with a paucity of red cell precursors. These criteria comprise "classical" DBA. The disorder results from a cellular defect, in which erythroid progenitors and precursors are highly sensitive to death by apoptosis, leading to erythropoietic failure [3-5].

DBA came into the scientific spotlight after the identification of RPS19, the gene encoding ribosomal protein S19 [6]. Since then, many genes that encode large (RPL) and small (RPS) ribosomal subunit proteins have been found to be mutated in DBA patients, such as RPL5, RPL11, RPL35A, RPS7, RPS10, RPS17, RPS24, and RPS26 [7-10].

Although the first case of DBA was reported in 1974 in Korea [11], there is relatively limited data regarding DBA. Recently, direct sequencing analysis of the RPS19 gene was performed with both cDNA and genomic DNA extracted from peripheral blood, and a c.3G>A point mutation in exon 2 resulting in a p.Met1Ile substitution was identified in a 3-month-old infant [12].

In this report, we reviewed the clinical characteristics of 56 patients with DBA from a 20-year birth cohort in Korea to provide further information for molecular analyses.

The medical records of 56 patients diagnosed with DBA were retrospectively reviewed from November 1984 to July 2010. Thirteen university hospitals participated in this study. Diagnostic and supporting criteria for the diagnosis of DBA are as follows: normochromic, often macrocytic anemia, reticulocytopenia, and a low number or lack of erythroid precursors in the bone marrow. In only two patients, diagnoses were confirmed by mutation analyses [12]. Clinical information, including records on birth history, age of onset and diagnosis, family history, height and weight, malformations, hematologic data, response to therapeutic procedures, and prognosis, was obtained via a standardized questionnaire. Remission was defined as a stable, physiologically acceptable hemoglobin (Hb) level maintained for at least 6 months independent of corticosteroids, transfusions, or other therapy.

Means and standard deviations were used in the statistical analysis. Statistical significance was defined as P<0.05.

The male to female ratio of patients was 1.67:1 (35 males, 21 females) as shown in Table 1. Among the patients diagnosed after 2000, the male-to-female ratio was 1.62:1 (21 males, 13 females). Only 3 cases (5.4%) revealed a family history. However, the genetic study was not done in the patients and family members.

The median age of diagnosis was 4 months, and the range was birth to 52 months of age. The proportion of patients diagnosed before 1 year was 74.1% (40/54), excluding 2 patients without age documents at diagnosis. From 2000 to 2009, 33 cases with DBA were registered, yielding an incidence of 6.6 cases per million, based on the Korean annual birth rate of 500,000. The mean Hb concentration was 5.0±1.8 g/dL (range, 1.5-9.8 g/dL), and the median was 5.2 g/dL. The mean value of the mean corpuscular volume (MCV) was 93.4±11.6 fL and mean fetal hemoglobin (HbF) level was 7.7±11.3%. The mean number of corrected reticulocytes was markedly lower than normal (19,700/mm³). The mean adenosine deaminase level was higher than normal, at 21.3±14.4 U/L. The mean cellularity of the bone marrow was 75%, and the myeloid:erythroid (M:E) ratio was 20.4:1 (range, 1.9:1 to 100:1). Among 28 patients with chromosomal analysis data, 26 showed a normal pattern in routine cytogenetic analysis. Direct sequencing analysis of the RPS19 gene was performed in 2 patients, who had the substitutions c.357-2A>G (RPS19) and c.3G>A (Met1Ile), respectively.

Excluding growth retardation, 21 cases (38.2%) showed congenital defects: thumb deformities (8 cases), ptosis (5), coarctation of aorta (5), ventricular septal defect (4), strabismus (3), cleft palate (2), and mitral valve prolapse (2). More than one anomaly was found in 21.2% of individuals (Table 2).

Thirty-two patients had a history of transfusion after the initial correction of Hb at diagnosis. All patients initially received corticosteroids, and 83.9% (47/56) responded with an increase in Hb. In 48.9% (23/47) of patients, steroids were eventually stopped with continued maintenance of adequate hemoglobin levels. The mean duration of steroid use was 7 years (maximum, 22 yrs). Complications due to steroid therapy were reported in 31 cases, including growth retardation (13), moon face (6), hirsutism (3), impaired glucose tolerance (3), and diabetes mellitus (2) (Table 3). Among the 28 patients who received steroids, 9 patients were on a well-controlled regimen, with a daily dose of 5 mg or less.

Iron chelation therapy was attempted in 6 patients with high serum ferritin levels (usually more than 1,000 ng/mL): deferoxamine (Desferal) intravenously in 3 cases, and more recently, deferasirox (Exjade) in 4 cases.

Five patients underwent hematopoietic stem cell transplantation (HSCT) at 81±46 months (range, 22-150 months). Transplantation sources were bone marrow from related donors (3 patients), bone marrow from unrelated donors (1 patient), and peripheral blood from unrelated donor (1 patient). Four recipients underwent conditioning without irradiation. Graft-versus-host disease prophylaxis included methotrexate (3 patients) and antithymocyte globulin (3 patients). All 5 patients have survived. Only one patient was lost to follow-up at the well-being state.

In the median follow-up of 8 years, there were 2 cases (3.6%) of malignancy: diffuse large cell lymphoma after 8 months and osteosarcoma after 13 years of DBA. The latter died due to lung metastasis and pulmonary hypertension.

DBA is characterized by macrocytic anemia, reticulocytopenia, and normal bone marrow cellularity with a paucity of erythroid precursors. DBA is caused by a defect in which erythroid progenitors and precursors are highly sensitive to death by apoptosis, leading to erythropoietic failure [4, 5, 13].

As in this study, routine cytogenetic analysis of patients with DBA is normal. However, a balanced reciprocal translocation t(X;19) was discovered in a DBA patient [6], revealing the first DBA gene (DBA1) that encodes a ribosomal protein S19 (RPS19) that is essential for the maturation of the 40S ribosomal subunit [14]. The identification of subsequent DBA genes has established DBA as a ribosomal disorder, because the affected gene encodes ribosomal proteins [8]. RPS19 protein haploinsufficiency is not sufficient to produce normal function. Recently, de novo mutations have been identified in ribosomal proteins RPS24 [8], RPS17, RPL5, RPL11, and RPL35a. To date, approximately 50% of DBA patients have a single mutation in a gene encoding a ribosomal protein. These findings implicate DBA as a disorder of ribosome biogenesis and/or function.

The diagnostic criteria for "classical" DBA, presented in 1976 [2], include the followings: anemia, usually presenting prior to the first birthday, with near normal, but variable, neutrophil and/or platelet counts; reticulocytopenia; macrocytosis; and normal marrow cellularity with a paucity of red cell precursors. "Non-classical" DBA is characterized by presentation at an age >1 year, with congenital anomalies, no anemia, a mild hematological phenotype (macrocytosis only), or the presence of a mutation shared by affected family members.

Although all immediate family members should be evaluated with a thorough relevant history, and a complete blood count, including red cell indices, erythrocyte adenosine deaminase (eADA) activity, and HbF, these were not completely performed in our study. This may be the reason why only 3 cases in our study revealed a family history. Generally, 40-45% of DBA cases are familial with autosomal dominant inheritance [15], and the remainder is sporadic or familial with seemingly different patterns of inheritance.

In a study of 80 DBA patients in the United Kingdom [16], 35% of classical DBA patients were found to have one or more unequivocal congenital anomalies, not including growth retardation. A similar proportion has been described in the French, Italian, and North American registries (40%, 46%, and 47%, respectively) [3, 17, 18]. More than one anomaly was found in up to 25% of individuals. In this study, 21 cases (38.2%) showed congenital defects, with more than one anomaly in 21.2%. Renal anomalies have not been seen. However, in the North American Diamond Blackfan Anemia Registry (DBAR), where the majority of patients undergo formal genitourinary and cardiac imaging, the prevalence is 19%.

The incidence of DBA is estimated to be between 1 in 100,000 and 1 in 200,000 without ethnic predilection, and both sexes are reported to be equally affected [3, 16-19]. In our study, the prevalence of DBA was similar to these statistics (6.6 cases per million), but with a male predilection (M:F=1.67:1).

The reported proportion of cancer is 4% (29/700) in patients with DBA, which is higher than the expected 0.5% for a cohort less than 30 years of age [20, 21]. The median age for cancer in these reports was 15 years, much younger than the median of 68 years in the general population [22]. The crude frequency reported from Boston Children's Hospital was 6.6% [23], the French series 3.8% [18], and the DBAR 1.9% (8/420) [3]. In this study, 2 cases (3.6%) with DBA developed cancer: osteogenic sarcoma and diffuse large cell lymphoma, respectively.

Corticosteroids remain the mainstay of DBA treatment. The therapeutic mechanism of corticosteroids in DBA may involve increased sensitivity to erythropoietin (Epo) [4, 24]. Remission occurred in 48.9%. The initial recommended starting dose was 2 mg/kg prednisone or the glucocorticoid equivalent. Most patients are controlled with the recommended maximum maintenance dose (≤1 mg/kg every other day or ≤0.5 mg/kg daily). Seventy percent of those who achieved remission did so within the first decade of life.

Complications due to steroid therapy were noted in 20% of patients on long-term steroid therapy [18], and 22% of those in the DBAR [3]. In our study, growth retardation was most common, followed by moon face, hirsutism, impaired glucose tolerance, and diabetes mellitus. Growth hormone therapy has been used successfully to increase growth velocity [25]. In our study, 1 patient who had been diagnosed with growth hormone deficiency showed good response to recombinant growth hormone therapy for 1.5 yrs (height increased from less than the 3rd percentile to the 10th percentile), without bone age advancement.

Addition of iron-chelating therapy is recommended in transfusion-dependent patients, because prolonged iron acquisition from transfusion can cause a potentially fatal iron overload in the liver, heart, or pancreas. Iron chelation was initiated when a ferritin level was 1,000 ng/mL or more instead of the hepatic iron concentration reaching 6-7 mg/g dry weight. Chelation by subcutaneous deferoxamine (Desferal, Novartis) was switched in 2007 to oral deferasirox (ICL670, Exjade, Novartis), which is now approved in Korea as the first-line therapy for transfusional iron overload [26].

Hematopoietic stem cell transplantation (HSCT) is the curative treatment for DBA [3, 18, 27, 28]. According to the DBAR [3], where the major indication for HSCT was transfusion dependence, the bone marrow was the most common source of stem cells. The survival rate for allogeneic sibling versus alternative donor transplant was 72.7% vs. 17.1% at >5 years following SCT. The survival for patients <10 years of age who underwent transplantation from human leukocyte antigen (HLA)-matched allogeneic siblings was 92.3% [19]. More recently, successful related umbilical cord blood and unrelated donor transplants have been reported to the DBAR, but it is too early for a meaningful analysis of the data.

In conclusion, the incidence of DBA is similar to data already published, but there was a male predilection in our study. Although all patients responded to initial treatment with steroids, about half needed further steroids after remission. It is necessary to collect further data, including information on management pathways, from nationwide DBA registries, in addition to molecular analyses. Since DBA patients need lifetime care, they should be managed by a multidisciplinary team involving pediatric and adult hematologists, internists, and subspecialists.

Table 1 Clinical and laboratory findings at diagnosis in 56 patients with Diamond Blackfan anemia.

Table 2 Congenital anomalies observed in 56 patients with Diamond Blackfan anemia.

Table 3 Side effects of steroid treatment in patients with Diamond Blackfan anemia.