Methylation patterns differed significantly between the patient and control groups. As shown in this figure, 24 out of 25 patients with B-ALL had an unmethylated pattern in their IKZF1 promoter genomic region, while this was 6 out of 25 normal cases. It shows a significant (P<0.05) unmethylation pattern in our patients with childhood B-ALL. On the other hand, it shows that most normal cases have methylated or partially methylated statues in their IKZF1 gene promoter site.