Time course experiments showing effects of activation period on neutrophil reaction against Der f extract. Measurements were performed from 0 min (just prior to addition) to 40 min after adding Der f extract in 10 min intervals (total of five times). The typical results of four separate experiments are shown. (A) Raw data of FSC and MFI values. The difference between stimulated values and unstimulated values was significant (P<0.05) starting at 10 min activation for both FSC and MFI. Asterisks indicate significant stimulated values. (B) Calculated indices (ΔFSC and NOI). The linearity of Der f-sNOI values according to the activation time was significant (P<0.01, asterisk), which was not observed for the ΔFSC values.

Abbreviations: Der f, Dermatophagoides farinae; FSC, forward scatter; MFI, mean fluorescence intensity; NOI, neutrophil oxidative index; SSC, side scatter.