(A) CE-HPLC of a case of Hb D-Iran/β-thalassemia showing a predominant peak in the Hb A2 window with area of 66.0% and retention time of 3.61 min with elevated Hb F with area of 21.3%. (B) CE-HPLC of a case of Hb D-Iran/Hb D-Punjab with two predominant peaks, the first in A2-window with area of 47.3%, and the second in the Hb D window comprising 41.4% with retention times 3.58 and 4.14 min, respectively.