Acute myeloid leukemia (AML) cells express and produce C-X-C chemokine receptor 7 (CXCR7). (A) Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in AML cell lines. (B) Western blot analysis in AML cell lines. (C) Flow cytometric analysis for CXCR7 in AML cell lines. (D) Flow cytometric analysis after dual staining of CXCR4 and CXCR7 in AML cell lines. (E) Flow cytometric analysis for CXCR7 in CD34⁺ cells obtained from the bone marrow of 5 patients with AML. (F) Immunofluorescence staining after permeabilization for CXCR4 and CXCR7 in U937 cells.

Abbreviations: CXCL12, C-X-C motif ligand 12; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.