86.3+/-6.5%).
Compared with control, deltaLNGFR transduction did not influence on the immunophenotype and cloning efficiency of CD34(+) cells. Among the deltaLNGFR(+)-derived colonies, 85.0% were shown to contain an integrated deltaLNGFR gene.
CONCLUSION: These data
show that retronectin and TPO-based cytokines can be used to facilitate retroviral transduction of CD34(+) cells. Also, deltaLNGFR transduced cells could be rapidly characterized by FACS analysis and effectively enriched by immunomagnetic selection
method. Further improvement of transduction conditions for stimulation of HSC proliferation without differentiation and development of new vectors that obviate the requirement for cell division are likely to enhance transduction of primitive HSC.